{"id":418,"date":"2021-05-27T12:06:57","date_gmt":"2021-05-27T10:06:57","guid":{"rendered":"http:\/\/www.life4fir.com\/?p=418"},"modified":"2021-05-27T12:06:57","modified_gmt":"2021-05-27T10:06:57","slug":"update-on-the-action-a-1-4-seed-conservation-at-low-temperature-18c-application-of-cryopreservation-protocols","status":"publish","type":"post","link":"http:\/\/www.life4fir.com\/es\/2021\/05\/27\/update-on-the-action-a-1-4-seed-conservation-at-low-temperature-18c-application-of-cryopreservation-protocols\/","title":{"rendered":"Actualizaci\u00f3n sobre la acci\u00f3n A 1.4 \u00abSeed conservation at low temperature (-18\u00b0C), application of cryopreservation protocols\u00bb"},"content":{"rendered":"

<\/p>\n

<\/div>\n

El proyecto LIFE4FIR est\u00e1 trabajando actualmente en la acci\u00f3n del proyecto A 1.4 ‘<\/span>Seed conservation at low temperature (-18\u00b0C), application of cryopreservation protocols\u2019.<\/p>\n

Las actividades relacionan el desarrollo de t\u00e9cnicas para la conservaci\u00f3n a bajas temperaturas de semillas, polen, embriones aislados y l\u00edneas de callos embriog\u00e9nicos de Abies nebrodensis<\/em>. Estas actividades son funcionales a la implementaci\u00f3n del banco de esperma y criobanco.<\/p>\n

 <\/p>\n

Cribado de semillas por rayos X.<\/h4>\n

El problema de las semillas vanas es el principal obst\u00e1culo en la germinaci\u00f3n de las semillas de Abies nebrodiensis<\/em>. El an\u00e1lisis de rayos X es una t\u00e9cnica desarrollada por IBE-CNR para la selecci\u00f3n r\u00e1pida de semillas con embri\u00f3n y semillas vac\u00edas de Abies nebrodensis<\/em> (Figura 1). Este procedimiento representa un cribado masivo de las semillas que se utilizar\u00e1n para las pruebas de germinaci\u00f3n o que se almacenar\u00e1n a -18 \u00b0 C.<\/p>\n

\"\"<\/p>\n

\"\"<\/p>\n

Figura 1 y Figura 2. Semillas maduras de Abies nebrodiensis observadas bajo rayos X para la selecci\u00f3n de semillas con embri\u00f3n (marcos superiores) y semillas vac\u00edas (marcos inferiores)<\/em><\/p>\n

\"\"<\/p>\n

Figura 3. Semillas maduras de Abies nebrodiensis con embri\u00f3n, vistas mediante rayos X<\/em><\/p>\n

 <\/p>\n

Prove di germinazione e coltura<\/h4>\n

Se realizaron pruebas de germinaci\u00f3n in vitro de semillas almacenadas a -18 \u00b0 C y embriones extra\u00eddos de semillas maduras y conservados en nevera (4 \u00b0 C) en los laboratorios CNR-IBE y Unipa-Saaf, colocando los embriones aislados en papel de filtro y luego en diferentes sustratos de cultivo (Figuras 4, 5 y 6).<\/p>\n

\"\"<\/p>\n

Figura 4. Aislamiento de embriones maduros de semillas de Abies nebrodensis<\/em><\/p>\n

\"\"<\/p>\n

Figura 5. Germinaci\u00f3n in vitro de un embri\u00f3n de Abies nebrodensis<\/em><\/p>\n

\"\"<\/p>\n

Figura 6. Germinaci\u00f3n y desarrollo in vitro de embriones de Abies nebrodensis<\/em><\/p>\n

 <\/p>\n

En los dos laboratorios se est\u00e1n realizando pruebas de cultivo in vitro de embriones inmaduros aislados de conos recolectados en julio, con el fin de obtener embriog\u00e9nesis directa o indirecta. El callo embriog\u00e9nico, obtenido de la escisi\u00f3n de los embriones extra\u00eddos de las semillas de los conos inmaduros de las plantas 6, 8, 10, 15, 16 y 27, muestra un crecimiento bastante lento. Se est\u00e1n probando varios medios de cultivo que contienen diferentes sales b\u00e1sicas y hormonas de crecimiento vegetal. Un mejor crecimiento de los callos parece verse favorecido por el aporte de citoquininas.<\/p>\n

Tambi\u00e9n se est\u00e1n realizando pruebas de germinaci\u00f3n en muestras de polen de Abies nebrodensis<\/em> almacenadas a bajas temperaturas (Fig. 7).<\/p>\n

\"\"<\/p>\n

Figura 7: grano de polen de Abies nebrodensis en la fase de germinaci\u00f3n<\/em><\/p>\n

<\/p>","protected":false},"excerpt":{"rendered":"

El proyecto LIFE4FIR est\u00e1 trabajando actualmente en la acci\u00f3n del proyecto A 1.4 ‘Seed conservation at low temperature (-18\u00b0C), application of cryopreservation protocols\u2019. Las actividades relacionan el desarrollo de t\u00e9cnicas para la conservaci\u00f3n a bajas temperaturas de semillas, polen, embriones aislados y l\u00edneas de callos embriog\u00e9nicos de Abies nebrodensis. Estas actividades son funcionales a la … <\/p>\n

Continuar leyendo \u00abActualizaci\u00f3n sobre la acci\u00f3n A 1.4 \u00abSeed conservation at low temperature (-18\u00b0C), application of cryopreservation protocols\u00bb\u00bb<\/span><\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[40],"tags":[],"yoast_head":"\nActualizaci\u00f3n sobre la acci\u00f3n A 1.4 "Seed conservation at low temperature (-18\u00b0C), application of cryopreservation protocols" - LIFE4FIR<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"http:\/\/www.life4fir.com\/es\/2021\/05\/27\/update-on-the-action-a-1-4-seed-conservation-at-low-temperature-18c-application-of-cryopreservation-protocols\/\" \/>\n<meta property=\"og:locale\" content=\"es_ES\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"[:it]Aggiornamento sull'Azione A 1.4 "Seed conservation at low temperature (-18\u00b0C), application of cryopreservation protocols"[:en]Update on the action A 1.4 "Seed conservation at low temperature (-18\u00b0C), application of cryopreservation protocols"[:es]Actualizaci\u00f3n sobre la acci\u00f3n A 1.4 "Seed conservation at low temperature (-18\u00b0C), application of cryopreservation protocols"[:] - LIFE4FIR\" \/>\n<meta property=\"og:description\" content=\"El proyecto LIFE4FIR est\u00e1 trabajando actualmente en la acci\u00f3n del proyecto A 1.4 ‘Seed conservation at low temperature (-18\u00b0C), application of cryopreservation protocols\u2019. Las actividades relacionan el desarrollo de t\u00e9cnicas para la conservaci\u00f3n a bajas temperaturas de semillas, polen, embriones aislados y l\u00edneas de callos embriog\u00e9nicos de Abies nebrodensis. Estas actividades son funcionales a la … Continuar leyendo "Actualizaci\u00f3n sobre la acci\u00f3n A 1.4 \u00abSeed conservation at low temperature (-18\u00b0C), application of cryopreservation protocols\u00bb"\" \/>\n<meta property=\"og:url\" content=\"http:\/\/www.life4fir.com\/es\/2021\/05\/27\/update-on-the-action-a-1-4-seed-conservation-at-low-temperature-18c-application-of-cryopreservation-protocols\/\" \/>\n<meta property=\"og:site_name\" content=\"LIFE4FIR\" \/>\n<meta property=\"article:published_time\" content=\"2021-05-27T10:06:57+00:00\" \/>\n<meta property=\"og:image\" content=\"http:\/\/www.life4fir.com\/wp-content\/uploads\/2021\/05\/Fig.-1-update-life-4-fir-life4fir-germination-300x300.png\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Escrito por\" \/>\n\t<meta name=\"twitter:data1\" content=\"adminl4f\" \/>\n\t<meta name=\"twitter:label2\" content=\"Tiempo de lectura\" \/>\n\t<meta name=\"twitter:data2\" content=\"6 minutos\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"WebSite\",\"@id\":\"http:\/\/www.life4fir.com\/it\/#website\",\"url\":\"http:\/\/www.life4fir.com\/it\/\",\"name\":\"LIFE4FIR\",\"description\":\"Decisive in situ and ex situ conservation strategies to secure the critically endangered Sicilian fir, Abies nebrodensis\",\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"http:\/\/www.life4fir.com\/it\/?s={search_term_string}\"},\"query-input\":\"required name=search_term_string\"}],\"inLanguage\":\"es\"},{\"@type\":\"ImageObject\",\"@id\":\"http:\/\/www.life4fir.com\/es\/2021\/05\/27\/update-on-the-action-a-1-4-seed-conservation-at-low-temperature-18c-application-of-cryopreservation-protocols\/#primaryimage\",\"inLanguage\":\"es\",\"url\":\"http:\/\/www.life4fir.com\/wp-content\/uploads\/2021\/05\/Fig.-1-update-life-4-fir-life4fir-germination.png\",\"contentUrl\":\"http:\/\/www.life4fir.com\/wp-content\/uploads\/2021\/05\/Fig.-1-update-life-4-fir-life4fir-germination.png\",\"width\":635,\"height\":632},{\"@type\":\"WebPage\",\"@id\":\"http:\/\/www.life4fir.com\/es\/2021\/05\/27\/update-on-the-action-a-1-4-seed-conservation-at-low-temperature-18c-application-of-cryopreservation-protocols\/#webpage\",\"url\":\"http:\/\/www.life4fir.com\/es\/2021\/05\/27\/update-on-the-action-a-1-4-seed-conservation-at-low-temperature-18c-application-of-cryopreservation-protocols\/\",\"name\":\"[:it]Aggiornamento sull'Azione A 1.4 \\\"Seed conservation at low temperature (-18\\u00b0C), application of cryopreservation protocols\\\"[:en]Update on the action A 1.4 \\\"Seed conservation at low temperature (-18\\u00b0C), application of cryopreservation protocols\\\"[:es]Actualizaci\\u00f3n sobre la acci\\u00f3n A 1.4 \\\"Seed conservation at low temperature (-18\\u00b0C), application of cryopreservation protocols\\\"[:] - LIFE4FIR\",\"isPartOf\":{\"@id\":\"http:\/\/www.life4fir.com\/it\/#website\"},\"primaryImageOfPage\":{\"@id\":\"http:\/\/www.life4fir.com\/es\/2021\/05\/27\/update-on-the-action-a-1-4-seed-conservation-at-low-temperature-18c-application-of-cryopreservation-protocols\/#primaryimage\"},\"datePublished\":\"2021-05-27T10:06:57+00:00\",\"dateModified\":\"2021-05-27T10:06:57+00:00\",\"author\":{\"@id\":\"http:\/\/www.life4fir.com\/it\/#\/schema\/person\/6dbcaac5052518d54f87023c8f4be778\"},\"breadcrumb\":{\"@id\":\"http:\/\/www.life4fir.com\/es\/2021\/05\/27\/update-on-the-action-a-1-4-seed-conservation-at-low-temperature-18c-application-of-cryopreservation-protocols\/#breadcrumb\"},\"inLanguage\":\"es\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"http:\/\/www.life4fir.com\/es\/2021\/05\/27\/update-on-the-action-a-1-4-seed-conservation-at-low-temperature-18c-application-of-cryopreservation-protocols\/\"]}]},{\"@type\":\"BreadcrumbList\",\"@id\":\"http:\/\/www.life4fir.com\/es\/2021\/05\/27\/update-on-the-action-a-1-4-seed-conservation-at-low-temperature-18c-application-of-cryopreservation-protocols\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Home\",\"item\":\"http:\/\/www.life4fir.com\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"Actualizaci\\u00f3n sobre la acci\\u00f3n A 1.4 \\u00abSeed conservation at low temperature (-18\\u00b0C), application of cryopreservation protocols\\u00bb\"}]},{\"@type\":\"Person\",\"@id\":\"http:\/\/www.life4fir.com\/it\/#\/schema\/person\/6dbcaac5052518d54f87023c8f4be778\",\"name\":\"adminl4f\",\"image\":{\"@type\":\"ImageObject\",\"@id\":\"http:\/\/www.life4fir.com\/it\/#personlogo\",\"inLanguage\":\"es\",\"url\":\"http:\/\/1.gravatar.com\/avatar\/a6e6c978c2825344b93af6f73f79ec98?s=96&d=mm&r=g\",\"contentUrl\":\"http:\/\/1.gravatar.com\/avatar\/a6e6c978c2825344b93af6f73f79ec98?s=96&d=mm&r=g\",\"caption\":\"adminl4f\"},\"url\":\"http:\/\/www.life4fir.com\/es\/author\/adminl4f\/\"}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"[:it]Aggiornamento sull'Azione A 1.4 \"Seed conservation at low temperature (-18\u00b0C), application of cryopreservation protocols\"[:en]Update on the action A 1.4 \"Seed conservation at low temperature (-18\u00b0C), application of cryopreservation protocols\"[:es]Actualizaci\u00f3n sobre la acci\u00f3n A 1.4 \"Seed conservation at low temperature (-18\u00b0C), application of cryopreservation protocols\"[:] - LIFE4FIR","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"http:\/\/www.life4fir.com\/es\/2021\/05\/27\/update-on-the-action-a-1-4-seed-conservation-at-low-temperature-18c-application-of-cryopreservation-protocols\/","og_locale":"es_ES","og_type":"article","og_title":"[:it]Aggiornamento sull'Azione A 1.4 \"Seed conservation at low temperature (-18\u00b0C), application of cryopreservation protocols\"[:en]Update on the action A 1.4 \"Seed conservation at low temperature (-18\u00b0C), application of cryopreservation protocols\"[:es]Actualizaci\u00f3n sobre la acci\u00f3n A 1.4 \"Seed conservation at low temperature (-18\u00b0C), application of cryopreservation protocols\"[:] - LIFE4FIR","og_description":"El proyecto LIFE4FIR est\u00e1 trabajando actualmente en la acci\u00f3n del proyecto A 1.4 ‘Seed conservation at low temperature (-18\u00b0C), application of cryopreservation protocols\u2019. Las actividades relacionan el desarrollo de t\u00e9cnicas para la conservaci\u00f3n a bajas temperaturas de semillas, polen, embriones aislados y l\u00edneas de callos embriog\u00e9nicos de Abies nebrodensis. Estas actividades son funcionales a la … Continuar leyendo \"Actualizaci\u00f3n sobre la acci\u00f3n A 1.4 \u00abSeed conservation at low temperature (-18\u00b0C), application of cryopreservation protocols\u00bb\"","og_url":"http:\/\/www.life4fir.com\/es\/2021\/05\/27\/update-on-the-action-a-1-4-seed-conservation-at-low-temperature-18c-application-of-cryopreservation-protocols\/","og_site_name":"LIFE4FIR","article_published_time":"2021-05-27T10:06:57+00:00","og_image":[{"url":"http:\/\/www.life4fir.com\/wp-content\/uploads\/2021\/05\/Fig.-1-update-life-4-fir-life4fir-germination-300x300.png"}],"twitter_card":"summary_large_image","twitter_misc":{"Escrito por":"adminl4f","Tiempo de lectura":"6 minutos"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"WebSite","@id":"http:\/\/www.life4fir.com\/it\/#website","url":"http:\/\/www.life4fir.com\/it\/","name":"LIFE4FIR","description":"Decisive in situ and ex situ conservation strategies to secure the critically endangered Sicilian fir, Abies nebrodensis","potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"http:\/\/www.life4fir.com\/it\/?s={search_term_string}"},"query-input":"required name=search_term_string"}],"inLanguage":"es"},{"@type":"ImageObject","@id":"http:\/\/www.life4fir.com\/es\/2021\/05\/27\/update-on-the-action-a-1-4-seed-conservation-at-low-temperature-18c-application-of-cryopreservation-protocols\/#primaryimage","inLanguage":"es","url":"http:\/\/www.life4fir.com\/wp-content\/uploads\/2021\/05\/Fig.-1-update-life-4-fir-life4fir-germination.png","contentUrl":"http:\/\/www.life4fir.com\/wp-content\/uploads\/2021\/05\/Fig.-1-update-life-4-fir-life4fir-germination.png","width":635,"height":632},{"@type":"WebPage","@id":"http:\/\/www.life4fir.com\/es\/2021\/05\/27\/update-on-the-action-a-1-4-seed-conservation-at-low-temperature-18c-application-of-cryopreservation-protocols\/#webpage","url":"http:\/\/www.life4fir.com\/es\/2021\/05\/27\/update-on-the-action-a-1-4-seed-conservation-at-low-temperature-18c-application-of-cryopreservation-protocols\/","name":"[:it]Aggiornamento sull'Azione A 1.4 \"Seed conservation at low temperature (-18\u00b0C), application of cryopreservation protocols\"[:en]Update on the action A 1.4 \"Seed conservation at low temperature (-18\u00b0C), application of cryopreservation protocols\"[:es]Actualizaci\u00f3n sobre la acci\u00f3n A 1.4 \"Seed conservation at low temperature (-18\u00b0C), application of cryopreservation protocols\"[:] - LIFE4FIR","isPartOf":{"@id":"http:\/\/www.life4fir.com\/it\/#website"},"primaryImageOfPage":{"@id":"http:\/\/www.life4fir.com\/es\/2021\/05\/27\/update-on-the-action-a-1-4-seed-conservation-at-low-temperature-18c-application-of-cryopreservation-protocols\/#primaryimage"},"datePublished":"2021-05-27T10:06:57+00:00","dateModified":"2021-05-27T10:06:57+00:00","author":{"@id":"http:\/\/www.life4fir.com\/it\/#\/schema\/person\/6dbcaac5052518d54f87023c8f4be778"},"breadcrumb":{"@id":"http:\/\/www.life4fir.com\/es\/2021\/05\/27\/update-on-the-action-a-1-4-seed-conservation-at-low-temperature-18c-application-of-cryopreservation-protocols\/#breadcrumb"},"inLanguage":"es","potentialAction":[{"@type":"ReadAction","target":["http:\/\/www.life4fir.com\/es\/2021\/05\/27\/update-on-the-action-a-1-4-seed-conservation-at-low-temperature-18c-application-of-cryopreservation-protocols\/"]}]},{"@type":"BreadcrumbList","@id":"http:\/\/www.life4fir.com\/es\/2021\/05\/27\/update-on-the-action-a-1-4-seed-conservation-at-low-temperature-18c-application-of-cryopreservation-protocols\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Home","item":"http:\/\/www.life4fir.com\/"},{"@type":"ListItem","position":2,"name":"Actualizaci\u00f3n sobre la acci\u00f3n A 1.4 \u00abSeed conservation at low temperature (-18\u00b0C), application of cryopreservation protocols\u00bb"}]},{"@type":"Person","@id":"http:\/\/www.life4fir.com\/it\/#\/schema\/person\/6dbcaac5052518d54f87023c8f4be778","name":"adminl4f","image":{"@type":"ImageObject","@id":"http:\/\/www.life4fir.com\/it\/#personlogo","inLanguage":"es","url":"http:\/\/1.gravatar.com\/avatar\/a6e6c978c2825344b93af6f73f79ec98?s=96&d=mm&r=g","contentUrl":"http:\/\/1.gravatar.com\/avatar\/a6e6c978c2825344b93af6f73f79ec98?s=96&d=mm&r=g","caption":"adminl4f"},"url":"http:\/\/www.life4fir.com\/es\/author\/adminl4f\/"}]}},"_links":{"self":[{"href":"http:\/\/www.life4fir.com\/es\/wp-json\/wp\/v2\/posts\/418"}],"collection":[{"href":"http:\/\/www.life4fir.com\/es\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/www.life4fir.com\/es\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/www.life4fir.com\/es\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/www.life4fir.com\/es\/wp-json\/wp\/v2\/comments?post=418"}],"version-history":[{"count":3,"href":"http:\/\/www.life4fir.com\/es\/wp-json\/wp\/v2\/posts\/418\/revisions"}],"predecessor-version":[{"id":429,"href":"http:\/\/www.life4fir.com\/es\/wp-json\/wp\/v2\/posts\/418\/revisions\/429"}],"wp:attachment":[{"href":"http:\/\/www.life4fir.com\/es\/wp-json\/wp\/v2\/media?parent=418"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/www.life4fir.com\/es\/wp-json\/wp\/v2\/categories?post=418"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/www.life4fir.com\/es\/wp-json\/wp\/v2\/tags?post=418"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}